Gaussian Modelling Characteristics of Peripheral Arterial Pulse: Difference between Measurements from the Three Trimesters of Healthy Pregnancy

Arterial pulse wave analysis has been attempted to monitor the maternal physiological changes of circulatory system during pregnancy. This study aimed to quantify the difference of Gaussian modelling characteristics derived from radial pulses measured from the three trimesters of healthy pregnant women. Radial pulses were recorded from seventy pregnant women between gestational week 11-13, week 20-22 and then week 37-39. They were then normalized and decomposed into three independent Gaussian waves for deriving four key modelling characteristic parameters: including the peak time interval (T) and peak amplitude ratio (R) between the first and second Gaussian waves (T1,2 and R1,2), and their corresponding values between the first and third Gaussian waves (T1,3 and R1,3). Post-hoc multiple comparisons after analysis of variance was then applied to study the within-subject differences in Gaussian modelling characteristics between the three trimesters. The key results were that T1,2 and T1,3 increased significantly (T1,2: 12.8±1.3 vs 13.2±1.3, p<0.05; T1,3: 39.5±4.3 vs 45.4±5.1, p<0.001), and R1,3 decreased significantly from the first to second trimester (0.60±0.15 vs 0.53±0.11, p<0.001). From the second to third trimester, T1,2 decreased significantly (13.2±1.3 vs 12.8±1.2, p<0.01), and T1,3 and R1,3 decreased slightly but non-significantly. Since larger T1,2 and T1,3 and smaller R1,3 are associated with more compliant peripheral arteries, our results indicated that peripheral arteries become more compliant from the first to second trimester and then have a tendency of returning to baseline during normal pregnancy. In conclusion, this study has quantitatively demonstrated significant changes of Gaussian modelling characteristics derived from radial pulses at the three trimesters of normal pregnant women, suggesting that these modelling characteristics could be used as parameters in monitoring maternal physiological changes during normal pregnancy

The impacts of water level fluctuations from paddy fields and aquaculture ponds on wetland habitats for wintering waterbirds: Implications for wetland management

The distribution and composition of wetland habitats for wintering waterbirds are heavily influenced by water level fluctuations. Through polder construction and aquaculture activities, paddy fields and aquaculture ponds have decreased the lateral connectivity of water level fluctuations in the lake. However, the impacts of water level fluctuations on habitat suitability, which can be seen using high-resolution images analysis, often cannot separate disturbances caused by the paddy fields and aquaculture ponds, and it is difficult to capture the actual impact of water level fluctuations on wetland habitats. Based on remote sensing image data and hydrological data, we selected Caizi Lake as a study site and comparatively analyzed the changes in wintering waterbird habitats in a water level sequence under the two scenarios. Our work showed that paddy fields and aquaculture ponds should be considered as potential options for creating more suitable habitats for migratory waterbirds if combined with reasonable and effective management of the water level within the paddy fields and aquaculture ponds. The present study results could facilitate the management and sustainable utilization of Caizi Lake wetlands and provide support for creating small habitats by managing the water levels of paddy fields and aquaculture ponds.This research was funded by the National Natural Science Foundation of China (Grant No. U2040210), the Scientific and Technology Project of the Anhui Provincial Group Limited for Yangtze-to-Huaihe Water Diversion (Grant No. YJJH-ZT-ZX-20180404062)

Arterial Pulse Waveform Characteristics Difference between the Three Trimesters of Healthy Pregnant Women

During pregnancy, the pregnant mother undergoes significant physiological changes in order to accommodate the developing fetus. In recent years, arterial pulse wave has been widely used to reflect these physiological changes. The aim of this study was to investigate the changes of radial pulse and photoplethysmography (PPG) pulse waveform characteristic with gestational age in normal pregnant women. 40 pregnant women volunteers were recruited from February 2016 to September 2016 from the Haidian Maternal Child Health Hospital in Beijing. Both radial pulses and PPG pulses were recorded simultaneously using a PowerLab data collection system at a sampling rate of 1000Hz for offline analysis. Their pulses were measured from each pregnant woman at three trimesters (first trimester between week 11-13; second trimester between week 20-22 and the third trimester between week 3739). Three waveform characteristics (total pulse area; pulse area1: the area before the notch position; pulse area2: the area after the notch position) were derived. The results showed that the total pulse area and pulse area2 from both radial and PPG pulses decreased significantly between two paired consecutive trimesters (all P<0.01, except the comparisons between the second and third trimesters for PPG pulses). In summary, this study has quantified the pulse waveform characteristic differences in terms of pulse areas between the three trimesters, providing useful scientific evidence to better understand the cardiovascular physiological changes during normal pregnancy

Changes of Arterial Pulse Waveform Characteristics with Gestational Age during Normal Pregnancy

Arterial pulse waveform analysis has been widely used to refect physiological changes in the cardiovascular system. This study aimed to comprehensively investigate the changes of waveform characteristics of both photoplethysmographic (PPG) and radial pulses with gestational age during normal pregnancy. PPG and radial pulses were simultaneously recorded from 130 healthy pregnant women at seven gestational time points. After normalizing the arterial pulse waveforms, the abscissa of notch point, the total pulse area and the refection index were extracted and compared between diferent measurement points and between the PPG and radial pulses using post-hoc multiple comparisons with Bonferrioni correction. The results showed that the efect of gestational age on all the three waveform characteristics was signifcant (all p<0.001) after adjusting for maternal age, heart rate and blood pressures. All the three waveform characteristics demonstrated similar changing trends with gestational age, and they were all signifcantly diferent between the measurements from gestational week 12–15 and the others (all p<0.05, except for the PPG total pulse area between the first and second measurement points). In conclusion, this study has comprehensively quantifed similar changes of both PPG and radial pulse waveform characteristics with gestational age

Muscleblind-Like 1 Knockout Mice Reveal Novel Splicing Defects in the Myotonic Dystrophy Brain

Myotonic dystrophy type 1 (DM1) is a multi-systemic disorder caused by a CTG trinucleotide repeat expansion (CTGexp) in the DMPK gene. In skeletal muscle, nuclear sequestration of the alternative splicing factor muscleblind-like 1 (MBNL1) explains the majority of the alternative splicing defects observed in the HSALR transgenic mouse model which expresses a pathogenic range CTGexp. In the present study, we addressed the possibility that MBNL1 sequestration by CUGexp RNA also contributes to splicing defects in the mammalian brain. We examined RNA from the brains of homozygous Mbnl1ΔE3/ΔE3 knockout mice using splicing-sensitive microarrays. We used RT-PCR to validate a subset of alternative cassette exons identified by microarray analysis with brain tissues from Mbnl1ΔE3/ΔE3 knockout mice and post-mortem DM1 patients. Surprisingly, splicing-sensitive microarray analysis of Mbnl1ΔE3/ΔE3 brains yielded only 14 candidates for mis-spliced exons. While we confirmed that several of these splicing events are perturbed in both Mbnl1 knockout and DM1 brains, the extent of splicing mis-regulation in the mouse model was significantly less than observed in DM1. Additionally, several alternative exons, including Grin1 exon 4, App exon 7 and Mapt exons 3 and 9, which have previously been reported to be aberrantly spliced in human DM1 brain, were spliced normally in the Mbnl1 knockout brain. The sequestration of MBNL1 by CUGexp RNA results in some of the aberrant splicing events in the DM1 brain. However, we conclude that other factors, possibly other MBNL proteins, likely contribute to splicing mis-regulation in the DM1 brain

Five-Year Longitudinal Assessment of the Downstream Impact on Schistosomiasis Transmission following Closure of the Three Gorges Dam

Schistosomiasis, caused by Schistosoma japonicum, is a significant parasitic disease and public health problem in China. How the parasite is transmitted there can be categorized into four distinct modes (modes I–IV) and it is predicted that the Three Gorges Dam, recently completed, will affect the way schistosomiasis is spread in these modes. We monitored transmission for a 5-year period (2002–2006) in eight villages, representative of the three modes (I–III) below the dam across four provinces (Hunan, Jiangxi, Hubei and Anhui) to determine whether there was any immediate impact of the dam on schistosomiasis spread. Human schistosomiasis incidence declined considerably within individual villages and each mode, and the yearly odds ratios (adjusted) for infection risk showed significant downward trends in all three modes over the follow-up period. The decreased human S. japonicum incidence recorded across transmission modes I–III was probably attributable to annual human and bovine praziquantel drug treatment. If an increase in schistosome transmission had occurred as a result of the dam, it would be of negligible size compared with this treatment-induced decline. There had thus been virtually no immediate impact of the TGD on schistosomiasis transmission downstream of the dam over the 5-year surveillance period